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By def-inition, primary culture indicates that the biological materialcomes directly from the tissue and is grown on a culture sur-face (e.g., dish or flask) without additional passaging.passaging is a process by which cells that have grown toconfluence (i.e., grown until they form a monolayer thatcompletely fills up the available surface) are removed fromtheir growth surface, diluted with culture medium, and thenreapplied to a new culture surface to continue to grow.Because many cellular processes change as a consequenceof culture conditions, many investigators prefer to use cellsin primary culture as these will exhibit properties andfunctions closest to those of the in vivo tissue. Pulsatile brain movement and associated hydrodynamics studied by magnetic reso-nance phase imaging.
Frequently, the PTA gives the pa-tient or a caregiver written instructions for exercises or activities that were taught during thetreatment session, such as a home exercise program (HEP).
If theexperimenter wishes to generalize only to people who will always have the same pretest,there is little problem with this factor. Transducer Ahas frequency of 7.5 MHz ultrasound beam diameter of 3 mm.
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